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The Slack channel is mainly expressed in Somatostatin positive neurons in the spinal cord. (A–F) Double immunofluorescence staining revealed the expression of the Slack channel ( A,D , in red) in somatostatin positive neurons ( B,E , in green). The ratio of somatostatin neurons with the Slack channel expression to total somatostatin neurons is 86% (10 sections, 967 neurons counted, 3 mice; C,F , in yellow). (G–L) Double immunofluorescence staining indicated the absence of Slack channel expression ( G,J , in red) in <t>dynorphin</t> positive neurons ( H,K , in green) in the spinal cord. The ratio of dynorphin positive neurons with the expression of Slack channel to total dynorphin positive neurons is 11 % (11 sections, 1123 cells counted, 3 mice; I,K , in yellow).
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The Slack channel is mainly expressed in Somatostatin positive neurons in the spinal cord. (A–F) Double immunofluorescence staining revealed the expression of the Slack channel ( A,D , in red) in somatostatin positive neurons ( B,E , in green). The ratio of somatostatin neurons with the Slack channel expression to total somatostatin neurons is 86% (10 sections, 967 neurons counted, 3 mice; C,F , in yellow). (G–L) Double immunofluorescence staining indicated the absence of Slack channel expression ( G,J , in red) in <t>dynorphin</t> positive neurons ( H,K , in green) in the spinal cord. The ratio of dynorphin positive neurons with the expression of Slack channel to total dynorphin positive neurons is 11 % (11 sections, 1123 cells counted, 3 mice; I,K , in yellow).
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Image Search Results


The Slack channel is mainly expressed in Somatostatin positive neurons in the spinal cord. (A–F) Double immunofluorescence staining revealed the expression of the Slack channel ( A,D , in red) in somatostatin positive neurons ( B,E , in green). The ratio of somatostatin neurons with the Slack channel expression to total somatostatin neurons is 86% (10 sections, 967 neurons counted, 3 mice; C,F , in yellow). (G–L) Double immunofluorescence staining indicated the absence of Slack channel expression ( G,J , in red) in dynorphin positive neurons ( H,K , in green) in the spinal cord. The ratio of dynorphin positive neurons with the expression of Slack channel to total dynorphin positive neurons is 11 % (11 sections, 1123 cells counted, 3 mice; I,K , in yellow).

Journal: Frontiers in Molecular Neuroscience

Article Title: The Slack Channel Deletion Causes Mechanical Pain Hypersensitivity in Mice

doi: 10.3389/fnmol.2022.811441

Figure Lengend Snippet: The Slack channel is mainly expressed in Somatostatin positive neurons in the spinal cord. (A–F) Double immunofluorescence staining revealed the expression of the Slack channel ( A,D , in red) in somatostatin positive neurons ( B,E , in green). The ratio of somatostatin neurons with the Slack channel expression to total somatostatin neurons is 86% (10 sections, 967 neurons counted, 3 mice; C,F , in yellow). (G–L) Double immunofluorescence staining indicated the absence of Slack channel expression ( G,J , in red) in dynorphin positive neurons ( H,K , in green) in the spinal cord. The ratio of dynorphin positive neurons with the expression of Slack channel to total dynorphin positive neurons is 11 % (11 sections, 1123 cells counted, 3 mice; I,K , in yellow).

Article Snippet: The following antibodies were used: mouse anti-slo2.2 (1:400; NeuroMab, clone N3/26), rabbit anti-calcitonin gene-related peptide (anti-CGRP; 1:800; Calbiochem), rabbit anti-NF200 (1:1000; Sigma-Aldrich, United States), rabbit anti-SOM (1:200, Thermo Fisher, United States), and rabbit anti-Dynorphin (1:200, Thermo Fisher, United States).

Techniques: Double Immunofluorescence Staining, Expressing

Schematic showing the pain gating control modification by the Slack channel knockout. “IB4 + ,” “SOM + ,” and “Dyn + ” represent the isolectin-B4, somatostatin, and dynorphin positive neurons, respectively. “T”: spinal pain signal transmission neuron. “+” and “–”represent excitatory and inhibitory roles in pain transmission, respectively. The red arrow indicated the enhanced activity due to the Slack channel knockout.

Journal: Frontiers in Molecular Neuroscience

Article Title: The Slack Channel Deletion Causes Mechanical Pain Hypersensitivity in Mice

doi: 10.3389/fnmol.2022.811441

Figure Lengend Snippet: Schematic showing the pain gating control modification by the Slack channel knockout. “IB4 + ,” “SOM + ,” and “Dyn + ” represent the isolectin-B4, somatostatin, and dynorphin positive neurons, respectively. “T”: spinal pain signal transmission neuron. “+” and “–”represent excitatory and inhibitory roles in pain transmission, respectively. The red arrow indicated the enhanced activity due to the Slack channel knockout.

Article Snippet: The following antibodies were used: mouse anti-slo2.2 (1:400; NeuroMab, clone N3/26), rabbit anti-calcitonin gene-related peptide (anti-CGRP; 1:800; Calbiochem), rabbit anti-NF200 (1:1000; Sigma-Aldrich, United States), rabbit anti-SOM (1:200, Thermo Fisher, United States), and rabbit anti-Dynorphin (1:200, Thermo Fisher, United States).

Techniques: Modification, Knock-Out, Transmission Assay, Activity Assay